SrasV1, Main, Exploration, bibRecord, 004D72

SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus

Identifieur interne : 004D72 ( Main/Exploration ); précédent : 004D71; suivant : 004D73

SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus

Auteurs : Patrick C. Y. Woo [Hong Kong] ; Susanna K. P. Lau [Hong Kong] ; Hoi-Wah Tsoi [Hong Kong] ; Zhi-Wei Chen [États-Unis] ; Beatrice H. L. Wong [Hong Kong] ; LINQI ZHANG [États-Unis] ; Jim K. H. Chan [Hong Kong] ; Lei-Po Wong [Hong Kong] ; WEI HE [République populaire de Chine] ; CHI MA [République populaire de Chine] ; Kwok-Hung Chan [Hong Kong] ; David D. Ho [États-Unis] ; Kwok-Yung Yuen [Hong Kong]

Source :

Vaccine [ 0264-410X ] ; 2005.

RBID : Pascal:05-0436229

Descripteurs français

English descriptors

KwdEn :
- Administration, Oral, Animals, Antibodies, Viral (blood), Antibodies, Viral (immunology), Bacterial Vaccines (administration & dosage), Bacterial Vaccines (immunology), Booster vaccination, Escherichia coli, Escherichia coli Proteins, Genetic vaccine, Immunization Schedule, Immunization, Secondary, Immunoglobulin G (blood), Injections, Intramuscular, Injections, Intraperitoneal, Interferon-gamma (analysis), Interleukin-4 (analysis), Male, Membrane Glycoproteins (administration & dosage), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Models, Animal, Neutralization Tests, Neutralizing antibody, Polypeptide, Recombinant DNA, SARS Virus (immunology), Severe Acute Respiratory Syndrome (prevention & control), Severe acute respiratory syndrome virus, Spike Glycoprotein, Coronavirus, Vaccines, DNA (administration & dosage), Vaccines, DNA (immunology), Vaccines, Synthetic (administration & dosage), Vaccines, Synthetic (immunology), Viral Envelope Proteins (administration & dosage), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
MESH :
- chemical , administration & dosage : Bacterial Vaccines, Membrane Glycoproteins, Vaccines, DNA, Vaccines, Synthetic, Viral Envelope Proteins.
- chemical , analysis : Interferon-gamma, Interleukin-4.
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Viral, Bacterial Vaccines, Membrane Glycoproteins, Vaccines, DNA, Vaccines, Synthetic, Viral Envelope Proteins.
- immunology : SARS Virus.
- prevention & control : Severe Acute Respiratory Syndrome.
- Administration, Oral, Animals, Escherichia coli Proteins, Immunization Schedule, Immunization, Secondary, Injections, Intramuscular, Injections, Intraperitoneal, Male, Mice, Mice, Inbred BALB C, Models, Animal, Neutralization Tests, Spike Glycoprotein, Coronavirus.

Abstract

Different forms of SARS coronavirus (SARS-CoV) spike protein-based vaccines for generation of neutralizing antibody response against SARS-CoV were compared using a mouse model. High IgG levels were detected in mice immunized with intraperitoneal (i.p.) recombinant spike polypeptide generated by Escherichia coli (S-peptide), mice primed with intramuscular (i.m.) tPA-optimize800 DNA vaccine (tPA-S-DNA) and boosted with i.p. S-peptide, mice primed with i.m. CTLA4HingeSARS800 DNA vaccine (CTLA4-S-DNA) and boosted with i.p. S-peptide, mice primed with oral live-attenuated Salmonella typhimurium (Salmonella-S-DNA-control) and boosted with i.p. S-peptide, mice primed with oral live-attenuated S. typhimurium that contained tPA-optimize800 DNA vaccine (Salmonella-tPA-S-DNA) and boosted with i.p. S-peptide, and mice primed with oral live-attenuated S. typhimurium that contained CTLA4HingeSARS800 DNA vaccine (Salmonella-tPA-S-DNA) and boosted with i.p. S-peptide. No statistical significant difference was observed among the Thl/Th2 index among these six groups of mice with high IgG levels. Sera of all six mice immunized with i.p. S-peptide, i.m. DNA vaccine control and oral Salmonella-S-DNA-control showed no neutralizing antibody against SARS-CoV. Sera of the mice immunized with i.m. tPA-S-DNA, i.m. CTLA4-S-DNA, oral Salmonella-S-DNA-control boosted with i.p. S-peptide, oral Salmonella-tPA-S-DNA, oral Salmonella-tPA-S-DNA boosted with i.p S-peptide, oral Salmonella-CTLA4-S-DNA and oral Salmonella-CTLA4-S-DNA boosted with i.p. S-peptide showed neutralizing antibody titers of <1:20-1:160. Sera of all the mice immunized with i.m. tPA-S-DNA boosted with i.p. S-peptide and i.m. CTLA4-S-DNA boosted with i.p. S-peptide showed neutralizing antibody titers of ≥1:1280. The present observation may have major practical value, such as immunization of civet cats, since production of recombinant proteins from E. coli is far less expensive than production of recombinant proteins using eukaryotic systems.

Affiliations:

Le document en format XML

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</author>
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</placeName>
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</affiliation>
</author>
<author><name sortKey="Linqi Zhang" sort="Linqi Zhang" uniqKey="Linqi Zhang" last="Linqi Zhang">LINQI ZHANG</name>
<affiliation wicri:level="2"><inist:fA14 i1="04"><s1>Aaron Diamond AIDS Research Center</s1>
<s2>NY</s2>
<s3>USA</s3>
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<author><name sortKey="Chan, Jim K H" sort="Chan, Jim K H" uniqKey="Chan J" first="Jim K. H." last="Chan">Jim K. H. Chan</name>
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<author><name sortKey="Wong, Lei Po" sort="Wong, Lei Po" uniqKey="Wong L" first="Lei-Po" last="Wong">Lei-Po Wong</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Room 423, University Pathology Building, Queen Mary Hospital</s1>
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</author>
<author><name sortKey="Wei He" sort="Wei He" uniqKey="Wei He" last="Wei He">WEI HE</name>
<affiliation wicri:level="3"><inist:fA14 i1="05"><s1>Peking Union Medical College</s1>
<s2>Beijing</s2>
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</placeName>
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<author><name sortKey="Chi Ma" sort="Chi Ma" uniqKey="Chi Ma" last="Chi Ma">CHI MA</name>
<affiliation wicri:level="3"><inist:fA14 i1="05"><s1>Peking Union Medical College</s1>
<s2>Beijing</s2>
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<sZ>9 aut.</sZ>
<sZ>10 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Room 423, University Pathology Building, Queen Mary Hospital</s1>
<s3>HKG</s3>
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<country>Hong Kong</country>
<wicri:noRegion>Department of Microbiology, The University of Hong Kong, Room 423, University Pathology Building, Queen Mary Hospital</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Centre of Infection and Immunology, Faculty of Medicine</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Research Centre of Infection and Immunology, Faculty of Medicine</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>11 aut.</sZ>
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</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ho, David D" sort="Ho, David D" uniqKey="Ho D" first="David D." last="Ho">David D. Ho</name>
<affiliation wicri:level="2"><inist:fA14 i1="04"><s1>Aaron Diamond AIDS Research Center</s1>
<s2>NY</s2>
<s3>USA</s3>
<sZ>4 aut.</sZ>
<sZ>6 aut.</sZ>
<sZ>12 aut.</sZ>
</inist:fA14>
<country>États-Unis</country>
<placeName><region type="state">État de New York</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, The University of Hong Kong, Room 423, University Pathology Building, Queen Mary Hospital</s1>
<s3>HKG</s3>
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<sZ>3 aut.</sZ>
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</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Department of Microbiology, The University of Hong Kong, Room 423, University Pathology Building, Queen Mary Hospital</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Research Centre of Infection and Immunology, Faculty of Medicine</s1>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>11 aut.</sZ>
<sZ>13 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Research Centre of Infection and Immunology, Faculty of Medicine</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong</s1>
<s3>HKG</s3>
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<wicri:noRegion>State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Vaccine</title>
<title level="j" type="abbreviated">Vaccine</title>
<idno type="ISSN">0264-410X</idno>
<imprint><date when="2005">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Vaccine</title>
<title level="j" type="abbreviated">Vaccine</title>
<idno type="ISSN">0264-410X</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Administration, Oral</term>
<term>Animals</term>
<term>Antibodies, Viral (blood)</term>
<term>Antibodies, Viral (immunology)</term>
<term>Bacterial Vaccines (administration & dosage)</term>
<term>Bacterial Vaccines (immunology)</term>
<term>Booster vaccination</term>
<term>Escherichia coli</term>
<term>Escherichia coli Proteins</term>
<term>Genetic vaccine</term>
<term>Immunization Schedule</term>
<term>Immunization, Secondary</term>
<term>Immunoglobulin G (blood)</term>
<term>Injections, Intramuscular</term>
<term>Injections, Intraperitoneal</term>
<term>Interferon-gamma (analysis)</term>
<term>Interleukin-4 (analysis)</term>
<term>Male</term>
<term>Membrane Glycoproteins (administration & dosage)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Models, Animal</term>
<term>Neutralization Tests</term>
<term>Neutralizing antibody</term>
<term>Polypeptide</term>
<term>Recombinant DNA</term>
<term>SARS Virus (immunology)</term>
<term>Severe Acute Respiratory Syndrome (prevention & control)</term>
<term>Severe acute respiratory syndrome virus</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vaccines, DNA (administration & dosage)</term>
<term>Vaccines, DNA (immunology)</term>
<term>Vaccines, Synthetic (administration & dosage)</term>
<term>Vaccines, Synthetic (immunology)</term>
<term>Viral Envelope Proteins (administration & dosage)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Administration par voie orale</term>
<term>Animaux</term>
<term>Anticorps antiviraux (immunologie)</term>
<term>Anticorps antiviraux (sang)</term>
<term>Calendrier vaccinal</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (administration et posologie)</term>
<term>Glycoprotéines membranaires (génétique)</term>
<term>Glycoprotéines membranaires (immunologie)</term>
<term>Immunoglobuline G (sang)</term>
<term>Injections musculaires</term>
<term>Injections péritoneales</term>
<term>Interféron gamma (analyse)</term>
<term>Interleukine-4 (analyse)</term>
<term>Modèles animaux</term>
<term>Mâle</term>
<term>Protéines Escherichia coli</term>
<term>Protéines de l'enveloppe virale (administration et posologie)</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Rappel de vaccin</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Syndrome respiratoire aigu sévère ()</term>
<term>Tests de neutralisation</term>
<term>Vaccins antibactériens (administration et posologie)</term>
<term>Vaccins antibactériens (immunologie)</term>
<term>Vaccins synthétiques (administration et posologie)</term>
<term>Vaccins synthétiques (immunologie)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Interferon-gamma</term>
<term>Interleukin-4</term>
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<term>Immunoglobulin G</term>
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<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term>
<term>Bacterial Vaccines</term>
<term>Membrane Glycoproteins</term>
<term>Vaccines, DNA</term>
<term>Vaccines, Synthetic</term>
<term>Viral Envelope Proteins</term>
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<term>Protéines de l'enveloppe virale</term>
</keywords>
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<term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Vaccins antibactériens</term>
<term>Vaccins synthétiques</term>
<term>Vaccins à ADN</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Immunoglobuline G</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Administration, Oral</term>
<term>Animals</term>
<term>Escherichia coli Proteins</term>
<term>Immunization Schedule</term>
<term>Immunization, Secondary</term>
<term>Injections, Intramuscular</term>
<term>Injections, Intraperitoneal</term>
<term>Male</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Models, Animal</term>
<term>Neutralization Tests</term>
<term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Administration par voie orale</term>
<term>Animaux</term>
<term>Calendrier vaccinal</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Injections musculaires</term>
<term>Injections péritoneales</term>
<term>Modèles animaux</term>
<term>Mâle</term>
<term>Protéines Escherichia coli</term>
<term>Rappel de vaccin</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Tests de neutralisation</term>
<term>Virus syndrome respiratoire aigu sévère</term>
<term>Escherichia coli</term>
<term>Polypeptide</term>
<term>Vaccin génétique</term>
<term>DNA recombinant</term>
<term>Rappel vaccination</term>
<term>Anticorps neutralisant</term>
</keywords>
</textClass>
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</teiHeader>
<front><div type="abstract" xml:lang="en">Different forms of SARS coronavirus (SARS-CoV) spike protein-based vaccines for generation of neutralizing antibody response against SARS-CoV were compared using a mouse model. High IgG levels were detected in mice immunized with intraperitoneal (i.p.) recombinant spike polypeptide generated by Escherichia coli (S-peptide), mice primed with intramuscular (i.m.) tPA-optimize800 DNA vaccine (tPA-S-DNA) and boosted with i.p. S-peptide, mice primed with i.m. CTLA4HingeSARS800 DNA vaccine (CTLA4-S-DNA) and boosted with i.p. S-peptide, mice primed with oral live-attenuated Salmonella typhimurium (Salmonella-S-DNA-control) and boosted with i.p. S-peptide, mice primed with oral live-attenuated S. typhimurium that contained tPA-optimize800 DNA vaccine (Salmonella-tPA-S-DNA) and boosted with i.p. S-peptide, and mice primed with oral live-attenuated S. typhimurium that contained CTLA4HingeSARS800 DNA vaccine (Salmonella-tPA-S-DNA) and boosted with i.p. S-peptide. No statistical significant difference was observed among the Thl/Th2 index among these six groups of mice with high IgG levels. Sera of all six mice immunized with i.p. S-peptide, i.m. DNA vaccine control and oral Salmonella-S-DNA-control showed no neutralizing antibody against SARS-CoV. Sera of the mice immunized with i.m. tPA-S-DNA, i.m. CTLA4-S-DNA, oral Salmonella-S-DNA-control boosted with i.p. S-peptide, oral Salmonella-tPA-S-DNA, oral Salmonella-tPA-S-DNA boosted with i.p S-peptide, oral Salmonella-CTLA4-S-DNA and oral Salmonella-CTLA4-S-DNA boosted with i.p. S-peptide showed neutralizing antibody titers of <1:20-1:160. Sera of all the mice immunized with i.m. tPA-S-DNA boosted with i.p. S-peptide and i.m. CTLA4-S-DNA boosted with i.p. S-peptide showed neutralizing antibody titers of ≥1:1280. The present observation may have major practical value, such as immunization of civet cats, since production of recombinant proteins from E. coli is far less expensive than production of recombinant proteins using eukaryotic systems.</div>
</front>
</TEI>
<affiliations><list><country><li>Hong Kong</li>
<li>République populaire de Chine</li>
<li>États-Unis</li>
</country>
<region><li>État de New York</li>
</region>
<settlement><li>Pékin</li>
</settlement>
</list>
<tree><country name="Hong Kong"><noRegion><name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C. Y." last="Woo">Patrick C. Y. Woo</name>
</noRegion>
<name sortKey="Chan, Jim K H" sort="Chan, Jim K H" uniqKey="Chan J" first="Jim K. H." last="Chan">Jim K. H. Chan</name>
<name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name>
<name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name>
<name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name>
<name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K. P." last="Lau">Susanna K. P. Lau</name>
<name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K. P." last="Lau">Susanna K. P. Lau</name>
<name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K. P." last="Lau">Susanna K. P. Lau</name>
<name sortKey="Tsoi, Hoi Wah" sort="Tsoi, Hoi Wah" uniqKey="Tsoi H" first="Hoi-Wah" last="Tsoi">Hoi-Wah Tsoi</name>
<name sortKey="Wong, Beatrice H L" sort="Wong, Beatrice H L" uniqKey="Wong B" first="Beatrice H. L." last="Wong">Beatrice H. L. Wong</name>
<name sortKey="Wong, Lei Po" sort="Wong, Lei Po" uniqKey="Wong L" first="Lei-Po" last="Wong">Lei-Po Wong</name>
<name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C. Y." last="Woo">Patrick C. Y. Woo</name>
<name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C. Y." last="Woo">Patrick C. Y. Woo</name>
<name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
<name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
<name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
</country>
<country name="États-Unis"><region name="État de New York"><name sortKey="Chen, Zhi Wei" sort="Chen, Zhi Wei" uniqKey="Chen Z" first="Zhi-Wei" last="Chen">Zhi-Wei Chen</name>
</region>
<name sortKey="Ho, David D" sort="Ho, David D" uniqKey="Ho D" first="David D." last="Ho">David D. Ho</name>
<name sortKey="Linqi Zhang" sort="Linqi Zhang" uniqKey="Linqi Zhang" last="Linqi Zhang">LINQI ZHANG</name>
</country>
<country name="République populaire de Chine"><noRegion><name sortKey="Wei He" sort="Wei He" uniqKey="Wei He" last="Wei He">WEI HE</name>
</noRegion>
<name sortKey="Chi Ma" sort="Chi Ma" uniqKey="Chi Ma" last="Chi Ma">CHI MA</name>
</country>
</tree>
</affiliations>
</record>

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SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus

SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus

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